Fluorescence lifetime imaging by using time-gated data acquisition
نویسندگان
چکیده
منابع مشابه
Fluorescence lifetime imaging by using time-gated data acquisition.
The use of the time gating technique for lifetime reconstruction in the Fourier domain is a novel technique. Time gating provides sufficient data points in the time domain for reliable application of the Fourier transform, which is essential for the time deconvolution of the system of the integral equations employed in the reconstruction. The Fourier domain telegraph equation is employed to mod...
متن کاملHigh-speed wide-field time-gated endoscopic fluorescence-lifetime imaging.
We report the development of a high-speed wide-field fluorescence-lifetime imaging (FLIM) system that provides fluorescence-lifetime images at rates of as many as 29 frames/s. A FLIM multiwell plate reader and a potentially portable FLIM endoscopic system operating at 355-nm excitation have been demonstrated.
متن کامل2-D fluorescence lifetime imaging using a time-gated image intensifier
We report a 2-D fluorescence lifetime imaging system based on a time-gated image intensifier and a Cr:LiSAP regenerative amplifier. We have demonstrated 185 ps temporal resolution. The deleterious effects of optical scattering are demonstrated. In recent years optical imaging in the visible/near infrared spectral region has been investigated as an altemative to traditional medical diagnostic te...
متن کاملFluorescence lifetime imaging by time-correlated single-photon counting.
We present a time-correlated single photon counting (TCPSC) technique that allows time-resolved multi-wavelength imaging in conjunction with a laser scanning microscope and a pulsed excitation source. The technique is based on a four-dimensional histogramming process that records the photon density over the time of the fluorescence decay, the x-y coordinates of the scanning area, and the wavele...
متن کاملTiming and Operating Mode Design for Time-Gated Fluorescence Lifetime Imaging Microscopy
Steady-state fluorence imaging and time-resolved fluorescence imaging are two important areas in fluorescence imaging research. Fluorescence lifetime imaging is an absolute measurement method which is independent of excitation laser intensity, fluorophore concentration, and photobleaching compared to fluorescence intensity imaging techniques. Time-gated fluorescence lifetime imaging microscopy ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied Optics
سال: 2007
ISSN: 0003-6935,1539-4522
DOI: 10.1364/ao.46.007384